August 15, 2014
I've decided to start a lab notebook using Evernote, please look there for updates:
https://www.evernote.com/pub/catherinem118/firstnotebook#st=p&n=d85686d3-0854-48e3-a133-2eb9db326a5b

August 12, 2014
I was able to meet with Carolyn last Friday, and I also had a work meeting on the system with Molly today. Molly and I will meet again this Thursday; she will be busy next week and free the week after that. Today I rearranged the filters on our system, but there is some confusion from different sources as to how best to order the jacuzzi and bio filters - I plan to follow up on this question with an email to our team tomorrow. However, the confusion / misinformation about the filters prevented me from adding water to the system today. Despite that set back, Molly and I started tested our four durafet probes : two work, one is unknown, and the probe that we suspected might not work (tested also during Laura Newcomb's 3 species mussel exp and labled with a "?" ) is in fact broken. The honeywell analyzer that we have in the basement seems to work alright so far, but the second analyzer is still M.I.A. - hopefully it is up at FHL OAEL. Tomorrow I will write to FHL to confirm the order of the filters, and ask if they would please send us a regulator that will read up to 30 PSI (we only have one that measures to 15 PSI right now). Next week I plan to add a UV filter (light + housing + other holder piece?) to our jacuzzi filter, add a charcoal bag to the biofilter (recommended by Sam and Bryanda) and begin to hook up the carbon dioxide gas to the analyzer. Molly explained that there will eventually be a card that puts the anazlyzer online, but that we might need to down load the labview program. I plan to meet with a few people this week and next week to ask their advice about building systems, and also to fine tune how our next set up will run - we need to decide which water tank will be pumped to our experimental chambers and how the sump / CO2 mixing will relate (esp when we move this system to Muk). I plan to move my labnote book to evernote or a similar tool kit, and will post a link to that site as soon as I am able. CM

August 5th, 2014

I had a meeting with Molly today and we began doing some work that we will finish on Thursday. The next steps will be to fit the system with push to connect tubing (for the water out flow from the tank to the sump) and fill the system with water to see if there are leaks. We will test the honeywell compiler and the probes to make sure they are working well before we procee. I put a switch after the "T from the chiller at the inflow to the tank - but eventually it would be good to replace that tubing and switch with something smaller to have more control over the water flow there. CM

Feeding Oysters:
We have 500 or so small 2-3" oysters sitting in two 2-3 gallon bucket, waiting to go into an experimental system.
Today I am feding them "enough (concentrated reed's shell fish diet) to make the tanks a light green...like green tea." from C.Friedman
Approx 9 mL for all oysters is my guess, 5 mL for a 3 gal bucket holding about 250 small 2-3" oysters . CM
  • UPDATE: Agust 11, 2014
Oysters should be fed about as much as they can clear in an hour or so. 2-3mL in this case. more informations is available on the shellfish diet website.

OA System:
This is the latest blueprint of our OA system, the basement lab edition. This system is not drawn to scale, and our goal is make the sump out of the largest tank available to us. In the basement there is a large cylindrical tank with a lid - could be a nice element to help contain the CO2. Part of this system, the mixing tank, will eventually be broken apart and sent to another lab to be used to condition adult shellfish during the winter. CM
OA system_August 1, 2014_CM.JPG

Parts:
Push to connect tubing info sent to me by Molly


August 4th, 2014
Now reading:
As Oysters Die, Climate Policy Goes on the Stump. By Coral Davenport, August 3rd, 2014 New York Times
http://mobile.nytimes.com/2014/08/04/us/as-oysters-die-climate-policy-goes-on-stump.html?smid=tw-share&_r=2&referrer

Reading:
Marine industries at risk on both coasts as oceans acidify. By Reid Wilson, July 30, 2014 Washington Post
http://www.washingtonpost.com/blogs/govbeat/wp/2014/07/30/marine-industries-at-risk-on-both-coasts-as-oceans-acidify/
CM

July 31, 2014
We got our order of 3/4" tubing so I added a " T " at the line out from the chiller so that some water will enter the experiment tank (controlled by a switch or valve) and some water will be diverted to the sump. In order to regulate temperature we've decided to put the pump outside the experimental tank, instead of inside. Water will circulate from the tank out to the pump and be pushed back in through a venturi injector (CO2 addition will take place there). CM

July 29, 2014
Things are progressing and we have a new design for the system - I will add it here asap. On Thursday Molly and I went to the shop and begun drilling holes for two tanks: one sump and one for the experimental organisms (this is also where the CO2 gas is mixed in). I have invited Molly to join my labnote book, since we are working together on this OA system, and she will soon be able to make additions here as well.

July 23, 2014

Now reading :

Novel microcosm system for investigating the effects of elevated carbon dioxide and temperature on intertidal organisms Helen S. Findlay, Michael A. Kendall, John I. Spicer, Carol Turley, Stephen WiddicombeFrom Aquatic Biology, August 2008
July 21, 2014 Here is a collaborative blue print that Molly and I made of the OA system. Flow rate to chiller will likely be around 240 - 960 gph (min - max). We've just ordered more flexible tubing to finish construction of the preliminary set up.
OA system.JPG

July 16, 2014
Yesterday, Tuesday, I did the basement lab check (equipment and pH, amonia water chemistry is done only on MWF), and fed the abolone some algae. Today Bryanna trained me on the protocol to change and clean the filters (charcoal, jacuzzi and bio-filters). Tomorrow we will do water changes in the basement tanks.
In addition to learning how to care for the abalone, I spent time building the OA system. I will soon add our current blueprint to this lab notebook. After a small amount of work in the shop tomorrow, I expect the OA system will be ready for a test run with instant ocean.
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I still need to look up chiller capacities, and ask Sam to order more tubing.

July 11, 2014 / Notes for basement OA system
Ideas for set up from conversation with Molly Roberts
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H20 input from same source ( this will help prevent pseudo replication problems )
1 chiller
1 set of filters
2 treatments
2 pCO2 inputs
2 venturi injectors
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look up chiller capacities
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Experimental parameters
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17 - 25 C
larvae, possibly adult organisms
UPDATE on July 14th, 2014, info from C.Friedman
Temps would range from 12 to 25
flow rates ideally would accommodate 3-4 gpm for each pH level.
Yes on shellfish: adults and larvae and juvs.